《植物生理学报》 2021, 57(1): 129-138

雌雄青杨土壤微生物遗产对川滇柳形态、 生长和光合能
力的影响

鲜婷¹，刘俊雁^1,2，文小梅¹，胥晓^1,2，董廷发^1,2,3,*

¹西华师范大学生命科学学院, 四川南充637009；²西南野生动植物资源保护教育部重点实验室, 四川南充637009；³四川省环境科学与生物多样性保护重点实验室, 西华师范大学西南山地特色植物种质适应与利用研究所, 四 川南充637009

通信作者：董廷发；E-mail: dongfar@163.com; dongtf@aliyun.com

摘　要：

植物土壤微生物遗产效应近年来开始受到关注, 这种效应是否与植株的性别有关还不得而知。本文以同域分布的青杨(Populus cathayana)和川滇柳(Salix rehderiana)为研究对象, 利用雌雄青杨土壤在未灭菌和灭菌条件下种植川滇柳, 比较川滇柳幼苗形态、生长、光合能力、叶氮含量对不同性别青杨的土壤及其灭菌条件的响应差异。结果显示: (1)种植于未灭菌雌性青杨土中的川滇柳生物量、光饱和光合速率(A)、光系统II有效光化学量子产量(Φ)、相对电子传递速率(ETR)、光合氮利用效率(PNUE)显著低于雄性青杨土中的川滇柳; 在灭菌的青杨土壤中, 川滇柳的上述性状对雌雄青杨的响应无显著差异。 (2)种植于青杨未灭菌土中的川滇柳高度、基茎、生物量、比根长、根比表面积和光合能力(A、气孔导度、蒸腾速率、 Φ、 ETR、 PNUE和叶绿素含量)比种植于青杨灭菌土中的川滇柳更低。这些结果表明雌雄青杨土壤的微生物遗产对川滇柳有不同的影响, 雌株的土壤遗产对川滇柳的生长和光合能力的抑制效应比雄株大。该结果从植物性别的角度认识植株土壤微生物遗产的效应, 为分析植物种间关系机理提供新的视角。

关键词：土壤微生物遗产效应; 生长; 光合能力; 雌雄异株; 川滇柳; 青杨

收稿：2020-06-24   修定：2020-12-02

资助：四川省科学技术厅重点研发项目(2019YFS0464)

Effects of soil microbial legacy of female and male Populus cathayana on morphology, growth and photosynthesis of Salix rehderiana

XIAN Ting¹, LIU Junyan^1,2, WEN Xiaomei¹, XU Xiao^1,2, DONG Tingfa^1,2,3,*

¹School of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, China; ²Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), Nanchong, Sichuan 637009, China; ³Key Laboratory of Environmental Science and Biodiversity Conservation (Sichuan Province), Institute of Plant Adaptation and Utilization in Southwest Mountains, China West Normal University, Nanchong, Sichuan 637009, China

Corresponding author: DONG Tingfa; E-mail: dongfar@163.com; dongtf@aliyun.com

Abstract:

In recent years, more attention has been paid to the effects of soil microbial legacy on plant–plant interactions. However, it remains unclear how sexual differences may impact these effects. In this study, we cultivated Salix rehderiana cuttings in the soils of female and male Populus cathayana with and without soil sterilization. We investigated the morphology, growth, photosynthesis and leaf nitrogen content of S. rehderiana in response to the different sex-based soil legacies of P. cathayana. The results show that: (1) biomass accumulation, light-saturated photosynthetic rate (A), effective photochemical quantum yield of photosystem II (Φ), relative photosynthetic electron transfer rate (ETR), and photosynthetic nitrogen utilization efciency (PNUE) of S. rehderiana cultivated in non-sterilized soil from female P. cathayana were lower, respectively, than those grown in male-associated soil. While there is no signifcant difference in above indices between S. rehderiana cultivated in female and male P. cathayana sterilized soils. (2) Compared to S. rehderiana grown in sterilized soil, those cultivated in non-sterilized soil had lower height, basal diameter, biomass accumulation, specifc root length, specifc root surface area and photosynthetic capacities (A, stomatal conductance, transpiration rate, Φ, ETR, PNUE, and chlorophyll content). Our results indicate that there is a sexual different effect of soil microbial legacy of P. cathayana on S. rehderiana, and the growth and photosynthesis of S. rehderiana suffer greater suppression when affected by the microbial soil legacy of female P. cathayana than male. These results provide new insights on the mechanism of interspecifc plant interactions by investigating plant sex-related soil microbial legacy effect.

Key words: soil microbial legacy effect; growth; photosynthetic capacity; dioecy; Salix rehderiana; Populus cathayana